Yes, it most certainly can. It's a bit strong, in my opinion, so go lightly. Put a thick buttercream dam around the edge, fill in with pie filling, and put the next layer on. I like to put a layer of whipped frosting on first, something light, and then the dam, and then the lemon. Very tasty!
I believe you can use lemon pie filling but I think that lemon curd may be a better choice because it sets a little firmer than the pie filling. If you do go with the pie filling..make sure you have a thick icing dam so that it won't spill out from between the layers.
HTH
You can thicken it up by adding a little cornstarch. But you'll need to heat it & mix the cornstarch w/ water before adding it to the pie filling. A curd you'll definitely need to refrigerate the cake.
I would go with lemon curd. It is definetly thicker. Lemon curd can be shelved when it is has been canned, but once open, it needs to be refigerated.
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